+ WHY IS BIOREACTOR TECHNOLOGY BETTER FOR MICROPROPAGATION?

Compared to traditional tissue culture techniques, bioreactor systems offer several advantages; they are time and labor saving, relatively easy to scale-up, allow enhanced growth and multiplication (e.g. by forced aeration) and improved nutrient availability due to the use of liquid medium.

Bioreactor-based plant propagation can increase the speed of multiplication and growth of cultures when commencing commercial propagation.

Ref 2: Plant Tissue Culture: Current Status and Opportunities > Altaf Hussain, Iqbal Ahmed Qarshi, Hummera Nazir and Ikram Ullah

http://cdn.intechopen.com/pdfs-wm/40180.pdf

+ ARE MICROPROPAGATED STARTER PLANTS GENETICLY IDENTICAL?

Differences between Cannabis Mother Plant and Cannabis plants produced using tissue culture, were statistically insignificant. (Pn = 1.23, gCO2 = 8.65, E = 0.46, Ci = 6.01, Ci /Ca = 0.04 and WUE = 0.41; data represents the least significant difference between cannabis mother plant and cannabis invitro procuded plants at p < 0.05).

In a similar experiment Bag et al. (2000) have reported at par or slightly better performance of tissue culture raised plants than mother plant in terms of their photosynthetic characteristics and morphological parameters.

Ref: Lata, H., et al., In vitro mass propagation of Cannabis sativa L.: A protocol refinement using novel aromatic cytokinin meta-topolin and the assessment of eco-physiological, biochemical and genetic fidelity of micropropagated plants. J. Appl. Res. Med. Aromat. Plants (2016)

http://dx.doi.org/10.1016/j.jarmap.2015.12.001

+ CAN USING TISSUE CULTURE REMOVE PLANT PESTS?

“Because the tissues are isolated and raised in contained and sterile environments, tissue culture is a highly effectively method for producing pest and disease-free planting material.”

ref: Conway, G 2012, One Billion Hungry, Can We Feed The World? Cornell University Press, Ithaca and London.

+ HOW CAN ORDERING FROM MERISTEMATIC IMPROVE MY PRODCUTION CHAIN?

The main advantage that tissue culture technology offers is the ability to produce a significant number of high quality and uniform planting material rapidly that can be multiplied year-round under sterile conditions anywhere irrespective of the season or weather.

ref Pillay, M & Tenkouano, A (eds.) 2011, Banana Breeding: Progress and Challenges, CRC Press, Boca Raton, London & New York.

+ WHY DO TISSUE CULTURED PLANTS SHOW MORE VIGOR?

Research data & reference coming soon.

+ HAVE ANY OTHER COMMERCIAL CROPS SUCCESSFULLY APPLIED TISSUE CULTURE?

The horticulture of food and ornamental crops like Banana, Sugarcane, Rice, Orchids, Corn, Soy, Ferns and Tobacco have successfully used tissue culture technologies to make significant crop improvements in recent years.

http://cdn.intechopen.com/pdfs-wm/40180.pdf

http://dx.doi.org/10.1016/j.jarmap.2015.12.001